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Research


Field of research


  • Stem cell biology: mechanisms of cellular reprogramming and differentiation.
  • Medical biotechnology: gene and cell therapy in modulation of neovascularization and inflammation.
  • Vascular and muscle biology: mechanisms of angiogenesis and vasculogenesis, differentiation of satellite cells.
  • Cancer biology: mechanisms of tumor initiation, growth, metastasis and resistance to therapay.
  • Molecular mechanisms of Duchenne muscular dystrophy – role of heme oxygenase-1 and microRNAs.
  • Role of microRNAs in cardiovascular disorders, kidney fibrosis and tumor development.
  • Role of hypoxia, anti-oxidant genes and microRNAs in regulation of gene expression.

Scientific achievements


  • Demonstration of the novel mechanisms of myoblasts differentiation: role of heme oxygenase-1 (HO-1) in regulation of microRNAs expression.
  • Demonstration of the opposite effect of hypoxia-inducible factors (HIF-1 and HIF-2) on the angiogenic genes expression.
  • Demonstration of the involvement of nitric oxide (NO) and HO-1 in regulation of expression and activity of vascular endothelial growth factor (VEGF).
  • Demonstration of the significance of HO-1 in tumor growth and resistance to therapy.
  • Demonstration of the importance of HO-1 in wound healing.
  • Demonstration of the crucial role of HO-1 in stromal cell-derived factor-1 (SDF-1) mediated neovascularization and endothelial progenitor cells function.
  • Demonstration of the angiogenic effect of 3-hydroxy-3-methylglutarylo- coenzyme A inhibitors (statins).
  • Demonstration of PPAR-γ independent activity of prostaglandin J2 (PGJ2) in regulation of angiogenic genes expression.

Specialized equipment


Modern animal facility - operating in SPF standard; houses several strains of knockout and transgenic mice and rats, kept in individually ventilated cages (IVC). The facility is equipped in:

  • Laser Doppler System for blood flow measurement in animals
  • Vevo 2100 (VisualSonics) - micro-ultrasound system for high-resolution imaging of small animals
  • IVIS© Lumina II (Caliper Life Science) - imaging system for both fluorescent and bioluminescent imaging in vivo
  • scil Vet abc - hematology analyzer (different animal species can be analyzed)

Flow cytometry laboratory, equipped in:

  • BD LSRFortessa flow cytometer
  • MoFloTMXDP (Beckman Coulter) - high-speed cell sorte
  • ImageStreamX (Amnis Corporation) – system which integrates the features of flow cytometry and fluorescent microscopy combined with a modern system of image analysis

Histological laboratory, equipped in:

  • automated tissue processor
  • automated tissue embedding system
  • paraffin & crytostat microtome
  • automated staining system for paraffin sections
  • Laser Microdissection LMD7000 (Leica) for cutting specific structures form frozen and paraffin-embedded sections

Other equippments:

  • Chambers for hypoxic conditions/hypoxic incubator for cells culturing in low oxygen concentration
  • Bioanalyzer 2100 (Agilent) for checking the RNA integrity
  • Real-time PCR StepOnePlus (Applied Biosystem) for analysis of a gene expression profile on mRNA level, as well as microRNAs analysis
  • AmpliSpeed slide cycler (Beckman Coulter) for RT-PCR reaction from very small amount of samples (even one cell)
  • FlexMap3D (Luminex) for measurement of a dozen or so of cytokines/growth factors in small volume of sample (serum/plasma/medium)

Animal models, kept in modern animal facility (SPF)


  • miR-146a knockout mice;
  • miR-378a knockout mice;
  • mdx mice (dystrophin knockout);
  • HO-1 knockout mice;
  • Nrf2 knockout mice;
  • diabetic db/db mice;

Selected techniques


  • Flow cytometry & cell sorting.
  • Molecular in vivo imaging (Vevo 2100, IVIS© Lumina II, Laser Doppler).
  • Laser microdissection method.
  • Regulated gene expression: hypoxia-induced and tetracycline-induced expression systems, establishing of stably-transfected cell lines.
  • siRNA techniques & microRNAs technology.
  • Angiogenic assays in vitro: tube formation on matrigel, ring aortic assay, spheroid assay.
  • In vivo models: hind limb ischemia, wound healing, tumor inoculation, chemical carcinogenesis, mouse model of myocardial infarction.
  • iPS technology.
  • Gene cloning, construction of plasmids and viral vectors: retroviral, lentiviral, adenoviral, AAV.
  • Isolation and culture of primary cells: satellite cells, hematopietic stem cells, mesenchymal stromal cells, endothelial cells, renal proximal epithelial cells.

 

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Jagiellonian University Faculty of Biochemistry, Biophysics and Biotechnology Department of Medical Biotechnology